García-Sáez group - membrane biophysics

Publications

Group Publications

2021

  • Mitochondrial outer membrane permeabilization at the single molecule level. Dadsena S, Jenner A, García-Sáez AJ. Review Cell Mol Life Sci 2021 Feb 12. Online ahead of print. doi: 10.1007/s00018-021-03771-4. Abstract

2020

  • Ferroptotic pores induce Ca 2+ fluxes and ESCRT-III activation to modulate cell death kinetics. Pedrera L, Espiritu RA, Ros U, Weber J, Schmitt A, Stroh J, Hailfinger S, von Karstedt S, García-Sáez AJ. Cell Death Differ 2020 Dec 17. Online ahead of print.doi: 10.1038/s41418-020-00691-x. Abstract
  • The Mysteries around the BCL-2 Family Member BOK. Shalaby R, Flores-Romero H, and García-Sáez AJ. Biomolecules. 2020 Dec; 10(12): 1638. doi: 10.3390/biom10121638 Abstract
  • Lipids glue BAK dimers together. Flores-Romero H, García-Sáez AJ. Nat Struct Mol Biol. 2020 Nov;27(11):1003-1004. doi: 10.1038/s41594-020-00516-y Abstract
  • Pore formation in regulated cell death. Flores-Romero H, Ros U, Garcia-Saez AJ. EMBO J. 2020 Dec 1; 39(23): e105753. doi: 10.15252/embj.2020105753 Abstract
  • The BCL-2 family saga. García-Sáez AJ. Nat Rev Mol Cell Biol. 2020 Oct;21(10):564-565. doi: 10.1038/s41580-020-0276-2. Abstract
  • Apoptotic stress induces Bax-dependent, caspase-independent redistribution of LINC complex nesprins. Lindenboim L, Grozki D, Amsalem-Zafran AR, Peña-Blanco A, Gundersen GG, Borner C, Hodzic D, Garcia-Sáez AJ, Worman HJ, Stein R. Cell Death Discov. 2020; 6: 90. doi: 10.1038/s41420-020-00327-6 Abstract
  • Structural characterization of an Arf dimer interface: molecular mechanism of Arf-dependent membrane scission. Diestelkoetter-Bachert P, Beck R, Reckmann I, Hellwig A, Garcia-Saez A, Zelman-Hopf M, Hanke A, Nunes Alves A, Wade RC, Mayer MP, Wieland F. FEBS Lett. 2020 Jul;594(14):2240-2253. PMID: 32394429 DOI: 10.1002/1873-3468.13808 Abstract
  • Drp1 Modulates Mitochondrial Stress Responses to Mitotic Arrest. Peña-Blanco A, Haschka MD, Jenner A, Zuleger T, Proikas-Cezanne T, Villunger A, García-Sáez AJ. Cell Death Differ. 2020 Mar 19. Online ahead of print. PMID: 32203171 DOI: 10.1038/s41418-020-0527-y Abstract
  • A Lipid Perspective on Regulated Cell Death. Flores-Romero H, Ros U, García-Sáez AJ. Int Rev Cell Mol Biol. 2020;351:197-236. PMID: 32247580 DOI: 10.1016/bs.ircmb.2019.11.004. Abstract
  • Partners in Crime: The Interplay of Proteins and Membranes in Regulated Necrosis. Ros U, Pedrera L, Garcia-Saez AJ. Int J Mol Sci. 2020 Mar 31;21(7):2412. PMID: 32244433 PMCID: PMC7177786 DOI: 10.3390/ijms21072412 Abstract
  • Mitochondrial residence of the apoptosis inducer BAX is more important than BAX oligomerization in promoting membrane permeabilization. Kuwana T, King LE, Cosentino K, Suess J, Garcia-Saez AJ, Gilmore AP, Newmeyer DD. J Biol Chem. 2020 Feb 7;295(6):1623-1636. doi: 10.1074/jbc.RA119.011635. Epub 2020 Jan 3. Abstract

2019

  • MERLIN: A Novel BRET-based Proximity Biosensor for Studying mitochondria-ER Contact Sites. Hertlein V, Flores-Romero H, K Das K, Fischer S, Heunemann M, Calleja-Felipe M, Knafo S, Hipp K, Harter K, Fitzgerald JC, García-Sáez AJ. Life Sci Alliance. 2019 Dec 9;3(1):e201900600. PMID: 31818884 PMCID: PMC6910062 DOI: 10.26508/lsa.201900600 Print 2020 Jan. Abstract
  • The Incomplete Puzzle of the BCL2 Proteins. Flores-Romero H, García-Sáez AJ. Cells. 2019 Sep 29;8(10). pii: E1176. doi: 10.3390/cells8101176. PMID: 31569576
  • A new perspective on membrane-embedded Bax oligomers using DEER and bioresistant orthogonal spin labels. Teucher M, Zhang H, Bader V, Winklhofer KF, García-Sáez AJ, Rajca A, Bleicken S, Bordignon E. Sci Rep. 2019 Sep 10;9(1):13013. doi: 10.1038/s41598-019-49370-z. PMID: 31506457
  • Single event visualization of unconventional secretion of FGF2. Dimou E, Cosentino K, Platonova E, Ros U, Sadeghi M, Kashyap P, Katsinelos T, Wegehingel S, Noé F, García-Sáez AJ, Ewers H, Nickel W. J Cell Biol. 2019 Feb 4;218(2):683-699. doi: 10.1083/jcb.201802008. PMID: 30470711
  • BFL1 modulates apoptosis at the membrane level through a bifunctional and multimodal mechanism showing key differences with BCLXL. Flores-Romero H, Landeta O, Ugarte-Uribe B, Cosentino K, García-Porras M, García-Sáez AJ, Basañez G. Cell Death Differ. 2019 Oct;26(10):1880-1894. doi: 10.1038/s41418-018-0258-5. PMID: 30560933
  • Quantitative analysis of super-resolved structures using ASAP. Danial JSH, Garcia-Saez AJ. Nat Methods. 2019 Aug;16(8):711-714. doi: 10.1038/s41592-019-0472-1. PMID: 31263253
  • Dynein light chain binding determines complex formation and posttranslational stability of the Bcl-2 family members Bmf and Bim. Singh PK, Roukounakis A, Weber A, Das KK, Sohm B, Villunger A, Garcia-Saez AJ, Häcker G. Cell Death Differ. 2019 Jun 12. doi: 10.1038/s41418-019-0365-y. PMID: 31189926
  • MAVS-induced mitochondrial membrane remodeling. Flores-Romero H, García-Sáez AJ. FEBS J. 2019 Apr;286(8):1540-1542. doi: 10.1111/febs.14822. PMID: 30957952
  • Single event visualization of unconventional secretion of FGF2. Dimou E, Cosentino K, Platonova E, Ros U, Sadeghi M, Kashyap P, Katsinelos T, Wegehingel S, Noé F, García-Sáez AJ, Ewers H, Nickel W. J Cell Biol. 2019 Feb 4;218(2):683-699. doi: 10.1083/jcb.201802008. PMID: 30470711
  • Quantification of the Interactions Between BCL-2 Proteins by Fluorescence Correlation Spectroscopy. Murad F, Garcia-Saez AJ. Methods Mol Biol. 2019;1877:337-350. doi: 10.1007/978-1-4939-8861-7_20. PMID: 30536014
  • AFM to Study Pore-Forming Proteins. Unsay JD, García-Sáez AJ. Methods Mol Biol. 2019;1886:191-202. doi: 10.1007/978-1-4939-8894-5_10. PMID: 30374868

2018

  • Topology of active, membrane-embedded Bax in the context of a toroidal pore. Bleicken S, Assafa TE, Stegmueller C, Wittig A, Garcia-Saez AJ, Bordignon E. Cell Death Differ. 2018 Nov;25(10):1717-1731. doi: 10.1038/s41418-018-0184-6. PMID: 30185826
  • Scanning Fluorescence Correlation Spectroscopy for Quantification of the Dynamics and Interactions in Tube Organelles of Living Cells. Unsay JD, Murad F, Hermann E, Ries J, García-Sáez AJ. Chemphyschem. 2018 Oct 18. doi: 10.1002/cphc.201800705. PMID: 30335213
  • MIM through MOM: the awakening of Bax and Bak pores. Cosentino K, García-Sáez AJ. EMBO J. 2018 Sep 3;37(17). pii: e100340. doi: 10.15252/embj.2018100340. PMID: 30135068
  • Early activation of CD95 is limited and localized to the cytotoxic synapse. Sánchez MF, Murad F, Gülcüler Balta GS, Martin-Villalba A, García-Sáez AJ, Carrer DC. FEBS J. 2018 Aug;285(15):2813-2827. doi: 10.1111/febs.14518. PMID: 29797791
  • Drp1 polymerization stabilizes curved tubular membranes similar to those of constricted mitochondria. Ugarte-Uribe B, Prévost C, Das KK, Bassereau P, García-Sáez AJ. J. Cell Sci. 2018 Apr 19;132(4). pii: jcs208603. doi: 10.1242/jcs.208603. PMID: 29361534
  • Bax retrotranslocation potentiates Bcl-xL's antiapoptotic activity and is essential for switch-like transitions between MOMP competency and resistance. Hantusch A, Das KK, García-Sáez AJ, Brunner T, Rehm M. Cell Death Dis. 2018 Mar 22;9(4):430. doi: 10.1038/s41419-018-0464-6. PMID: 29567940
  • The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane. Böhler P, Stuhldreier F, Anand R, Kondadi AK, Schlütermann D, Berleth N, Deitersen J, Wallot-Hieke N, Wu W, Frank M, Niemann H, Wesbuer E, Barbian A, Luyten T, Parys JB, Weidtkamp-Peters S, Borchardt A, Reichert AS, Peña-Blanco A, García-Sáez AJ, Itskanov S, van der Bliek AM, Proksch P, Wesselborg S, Stork B. Cell Death Dis. 2018 Feb 19;9(3):286. doi: 10.1038/s41419-018-0312-8. PMID: 29459714
  • Bcl-2 proteins: Unraveling the details of a complex and dynamic network. Bleicken S, Garcia-Saez AJ. Mol Cell Oncol. 2017 Oct 30;5(1):e1384880. doi: 10.1080/23723556.2017.1384880. eCollection 2018. PMID: 29404386
  • Drp1 polymerization stabilizes curved tubular membranes similar to those of constricted mitochondria. Ugarte-Uribe B, Prévost C, Das KK, Bassereau P, García-Sáez AJ. J Cell Sci. 2018 Apr 19;132(4). pii: jcs208603. doi: 10.1242/jcs.208603. PMID: 29361534
  • Bax, Bak and beyond - mitochondrial performance in apoptosis. Peña-Blanco A, García-Sáez AJ. FEBS J. 2018 Feb;285(3):416-431. doi: 10.1111/febs.14186. Epub 2017 Sep 4. Review. PMID: 28755482

2017

  • Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis.Singh PK, Roukounakis A, Frank DO, Kirschnek S, Das KK, Neumann S, Madl J, Römer W, Zorzin C, Borner C, Haimovici A, Garcia-Saez A, Weber A, Häcker G. Genes Dev. 2017 Sep 1;31(17):1754-1769. doi: 10.1101/gad.302497.117. PMID: 28982759
  • Transient apoptosis inhibition in donor stem cells improves hematopoietic stem cell transplantation.Kollek M, Voigt G, Molnar C, Murad F, Bertele D, Krombholz CF, Bohler S, Labi V, Schiller S, Kunze M, Geley S, Niemeyer CM, Garcia-Saez A, Erlacher M. J Exp Med. 2017 Oct 2;214(10):2967-2983. doi: 10.1084/jem.20161721. Epub 2017 Sep 7. PMID: 28882984
  • Does Ceramide Form Channels? The Ceramide-Induced Membrane Permeabilization Mechanism. Artetxe I, Ugarte-Uribe B, Gil D, Valle M, Alonso A, García-Sáez AJ, Goñi FM. Biophys J. 2017 Aug 22;113(4):860-868. doi: 10.1016/j.bpj.2017.06.071. PMID: 28834722
  • Bax, Bak and beyond: mitochondrial performance in apoptosis. Peña-Blanco A, García-Sáez AJ. FEBS J. 2017 Jul 29. doi: 10.1111/febs.14186. [Epub ahead of print] Review.PMID:2875548
  • Quantitative interactome of a membrane Bcl-2 network identifies a hierarchy of complexes for apoptosis regulation.Bleicken S, Hantusch A, Das KK, Frickey T, Garcia-Saez AJ.Nat Commun. 2017 Jul 13;8(1):73. doi: 10.1038/s41467-017-00086-6.
  • Apoptotic foci at mitochondria: in and around Bax pores.Ugarte-Uribe B, García-Sáez AJ. Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726). pii: 20160217. doi: 10.1098/rstb.2016.0217. Review.PMID: 28630156
  • Improving certainty in single molecule imaging.Danial JS, García-Sáez AJ. Curr Opin Struct Biol. 2017 May 5;46:24-30. doi: 10.1016/j.sbi.2017.04.007. [Epub ahead of print] Review.PMID: 28482279
  • Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium. Ros U, Peña-Blanco A, Hänggi K, Kunzendorf U, Krautwald S, Wong WW, García-Sáez AJ. Cell Rep. 2017 Apr 4;19(1):175-187. doi: 10.1016/j.celrep.2017.03.024.
  • Determinants of BH3 Sequence Specificity for the Disruption of Bcl-xL/cBid Complexes in Membranes. Das KK, Shalaby R, García-Sáez AJ. ACS Chem Biol. 2017 Apr 21;12(4):989-1000. doi: 10.1021/acschembio.6b01084. Epub 2017 Feb 17. PMID: 28170214
  • The membrane activity of BOK involves formation of large, stable toroidal pores and is promoted by cBID. Fernández-Marrero Y, Bleicken S, Das KK, Bachmann D, Kaufmann T, Garcia-Saez AJ. FEBS J. 2017 Jan 8. doi: 10.1111/febs.14008. [Epub ahead of print] PMID:28064468
  • Pro-apoptotic cBid and Bax exhibit distinct membrane remodeling activities: An AFM study. Unsay JD, Cosentino K, Sporbeck K, García-Sáez AJ. Biochim Biophys Acta. 2016 Oct 15;1859(1):17-27. doi: 10.1016/j.bbamem.2016.10.007. [Epub ahead of print] PMID:27755971

2016

  • Disrupting a key hydrophobic pair in the oligomerization interface of the actinoporins impairs their pore-forming activity. Mesa-Galloso H, Delgado-Magnero KH, Cabezas S, López-Castilla A, Hernández-González JE, Pedrera L, Alvarez C, Tieleman DP, García-Saez AJ, Lanio ME, Ros U, Valiente PA. Protein Sci. 2016 Dec 21. doi: 10.1002/pro.3104. [Epub ahead of print] PMID:28000294
  • Bax and Bak Pores: Are We Closing the Circle? Cosentino K, García-Sáez AJ. Trends Cell Biol. 2016 Dec 5. pii: S0962-8924(16)30202-1. doi: 10.1016/j.tcb.2016.11.004. [Epub ahead of print] Review.PMID:27932064
  • Pushing the size limit of de novo structure ensemble prediction guided by sparse SDSL-EPR restraints to 200 residues: The monomeric and homodimeric forms of BAX. Fischer AW, Bordignon E, Bleicken S, García-Sáez AJ, Jeschke G, Meiler J. J Struct Biol. 2016 Jul;195(1):62-71. doi: 10.1016/j.jsb.2016.04.014.
  • MOMP in the absence of BH3-only proteins. García Sáez AJ, Villunger A. Genes Dev. 2016 Apr 15;30(8):878-80. doi: 10.1101/gad.281519.116.
  • Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs. Navarro G, Cordomí A, Zelman-Femiak M, Brugarolas M, Moreno E, Aguinaga D, Perez-Benito L, Cortés A, Casadó V, Mallol J, Canela EI, Lluís C, Pardo L, García-Sáez AJ, McCormick PJ, Franco R. BMC Biol. 2016 Apr 5;14:26. doi: 10.1186/s12915-016-0247-4.
  • cBid, Bax and Bcl-xL exhibit opposite membrane remodeling activities. Bleicken S, Hofhaus G, Ugarte-Uribe B, Schröder R, García-Sáez AJ. Cell Death Dis. 2016 Feb 25;7:e2121. doi: 10.1038/cddis.2016.34. PMID: 26913610
  • Bax assembly into rings and arcs in apoptotic mitochondria is linked to membrane pores. Salvador-Gallego R, Mund M, Cosentino K, Schneider J, Unsay J, Schraermeyer U, Engelhardt J, Ries J, García-Sáez AJ. EMBO J. 2016 Jan 18. pii: e201593384. [Epub ahead of print] PMID: 26783362

2015

  • Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol. Sudji IR, Subburaj Y, Frenkel N, García-Sáez AJ, Wink M. Molecules. 2015 Nov 9;20(11):20146-60. doi: 10.3390/molecules201119682. PMID:26569199 .
  • Bax monomers form dimer units in the membrane that further self-assemble into multiple oligomeric species. Subburaj Y, Cosentino K, Axmann M, Pedrueza-Villalmanzo E, Hermann E, Bleicken S, Spatz J, García-Sáez AJ. Nat Commun. 2015 Aug 14;6:8042. doi: 10.1038/ncomms9042.
  • Differences in activity of actinoporins are related with the hydrophobicity of their N-terminus . Ros U, Rodríguez-Vera W, Pedrera L, Valiente PA, Falcón SC, Lanio ME, García-Saez AJ, Alvarez C. Biochimie. 2015 Jun 29. pii: S0300-9084(15)00203-5. doi: 10.1016/j.biochi.2015.06.024. [Epub ahead of print] PMID:26134716
  • Minimalist model systems reveal similarities and differences between membrane interaction modes of MCL1 and BAK. Landeta O, Landajuela A, Garcia-Saez A, Basañez G. J Biol Chem. 2015 May 18. pii: jbc.M114.602193. [Epub ahead of print] PMID: 25987560
  • Formation of Disulfide Bridges Drives Oligomerization, Membrane Pore Formation and Translocation of Fibroblast Growth Factor 2 to Cell Surfaces. Müller HM, Steringer JP, Wegehingel S, Bleicken S, Münster M, Dimou E, Unger S, Weidmann G, Andreas H, García-Sáez AJ, Wild K, Sinning I, Nickel W. J Biol Chem. 2015 Feb 18. pii: jbc.M114.622456. [Epub ahead of print]

2014

  • Toxicity of an a-pore-forming toxin depends on the assembly mechanism on the target membrane as revealed by single-molecule imaging. Subburaj Y, Ros U, Hermann E, Tong R, Garcia-Saez AJ. J Biol Chem. 2015 Feb 20;290(8):4856-65. doi: 10.1074/jbc.M114.600676. Epub 2014 Dec 18 PMID: 25525270
  • Structural model of active Bax at the membrane. Bleicken S, Jeschke G, Stegmueller C, Salvador-Gallego R, Garcia-Saez AJ, Bordignon E Mol Cell. 2014 Nov 20;56(4):496-505. doi:10.1016/j.molcel.2014.09.022. Epub 2014 Oct 30. PMID: 25458844
  • Dynamin-Related Protein 1 (Drp1) Promotes Structural Intermediates of Membrane Division. Ugarte-Uribe B., Mueller HM., Otsuki M., Nickel W., Garcia-Saez AJ. J Biol Chem. 2014 Sept 18 [Epub ahead of print]; doi: 10.1074/jbc.M114.575779
  • Automated analysis of Giant Unilamellar Vesicles using Circular Hough Transformation. Hermann E., Bleicken S., Subburaj Y., García-Sáez AJ. Bioinformatics. 2014 Feb 18.;

2013

  • Proapoptotic Bax and Bak form stable protein-permeable pores of tunable size. Bleicken S, Landeta O, Landajuela A, Basañez G, García-Sáez AJ. J Biol Chem.2013 Nov 15; 288(46):33241-52. doi: 10.1074/jbc.M113.512087. Epub 2013 Oct 7. PMID: 24100034
  • Cardiolipin effects on membrane structure and dynamics. Unsay J, Cosentino K, Subburaj Y, García-Sáez AJ. Langmuir. 2013 Dec 23;29(51):15878-87. doi: 10.1021/la402669z. Epub 2013 Sep 10. PMID 23962277z
  • Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for Bid. Jalmar O, François-Moutal L, García-Sáez AJ, Perry M, Granjon T, Gonzalvez F, Gottlieb E, Ayala-Sanmartin J, Klösgen B, Schwille P, Petit PX. PLoS One. 2013;8(2):e55250. doi: 10.1371/journal.pone.0055250. Epub 2013 Feb 13. PubMed PMID: 23418437; PubMed Central PMCID: PMC3572128.
  • Mechanistic Differences in the Membrane Activity of Bax and Bcl-xL Correlate with Their Opposing Roles in Apoptosis. Bleicken S, Wagner C, and García-Sáez AJ. Biophys J. 2013 Jan; 104(2):421-431

2012

  • PI(4,5)P2 Dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. Steringer JP, Bleicken S, Andreas H, Zacherl S, Laussmann M, Temmerman K, Contreras FX, Bharat TA, Lechner J, Muller HM, Briggs JA, García-Sáez AJ, Nickel W.
  • Dynamic interaction of cBid with detergents, liposomes and mitochondria. Bleicken S, García-Sáez AJ, Conte E, Bordignon E.

2011

  • Oligomerization and pore formation by equinatoxin II inhibit endocytosis and lead to plasma membrane reorganization. García-Sáez AJ, Buschhorn SB, Keller H, Anderluh G, Simons K, Schwille P.
  • Destabilization exerted by peptides derived from the membrane-proximal external region of HIV-1 gp41 in lipid vesicles supporting fluid phase coexistence. Apellaniz B, García-Sáez AJ, Nir S, Nieva JL. BBA Biomembranes 2011 July 7; 1808(7):1797-1805.

2010

  • All-or-none vs. graded: single vesicle analysis evidences lipid composition effects on membrane permeabilization. Apellániz B, Nieva JL, Schwille P, García-Sáez AJ. Biophys J. 2010 Dec 1;99(11):3619-28.
  • Giant unilamellar vesicles (GUVs) as a new tool for analysis of caspase-8/Bid-FL complex binding to cardiolipin and its functional activity. Jalmar O, García-Sáez AJ, Berland L, Gonzalvez F, Petit PX. Cell Diff and Dis. 2010 1, e103; doi:10.1038/cddis.2010.81
  • A comprehensive framework for fluorescence correlation spectroscopy. Ries J, Petrásek Z, García-Sáez AJ, Schwille P. New J. Phys. 2010 Nov 12:113009
  • Pores formed by baxa5 relax to a smaller size and keep at equilibrium. Fuertes G, García-Sáez AJ, Esteban-Martín S, Giménez D, Sánchez-Muñoz OL, Schwille P, Salgado J. Biophysical journal. 2010 Nov 3;99(9):2917-25.
  • Permeabilization of the outer mitochondrial membrane by Bcl-2 proteins. García-Sáez AJ, Fuertes G, Suckale J, Salgado J. Adv Exp Med Biol. 2010;677:91-105. Review.
  • Role of membrane lipids for the activity of pore forming proteins and peptides. Fuertes G, Giménez D, Esteban-Martín S, García-Sáez AJ, Sánchez O, Salgado J. Adv Exp Med Biol. 2010;677:31-55. Review.
  • Yeast lipids can phase separate into micrometer-scale membrane domains. Klose C, Ejsing CS, Garcia-Saez AJ, Kaiser HJ, Sampaio JL, Surma MA, Shevchenko A, Schwille P, Simons K. The Journal of biological chemistry. 2010 Sep 24;285(39):30224-32.
  • Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids. Apellaniz B, García-Sáez AJ, Huarte N, Kunert R, Vorauer-Uhl K, Katinger H, Schwille P, Nieva JL. FEBS Lett. 2010 Apr 16;584(8):1591-6.
  • Stability of lipid domains. García-Sáez AJ, Schwille P. FEBS Lett. 2010 May 3;584(9):1653-8. Epub 2009 Dec 27. Review.
  • Fluorescence correlation spectroscopy for the study of membrane dynamics and organization in giant unilamellar vesicles. García-Sáez AJ, Carrer DC, Schwille P. Methods Mol Biol. 2010;606:493-508.

2009

  • Membrane promotes tBID interaction with BCL(XL). García-Sáez AJ, Ries J, Orzáez M, Pérez-Payà E, Schwille P. Nat Struct Mol Biol. 2009 Nov;16(11):1178-85.
  • Surface analysis of membrane dynamics. García-Sáez AJ, Schwille P. Biochim Biophys Acta. 2010 Apr;1798(4):766-76.
  • Detergent-activated BAX protein is a monomer. Ivashyna O, García-Sáez AJ, Ries J, Christenson ET, Schwille P, Schlesinger PH. J Biol Chem. 2009 Sep 4;284(36):23935-46.

2008

  • Fluorescence correlation spectroscopy for the study of membrane dynamics and protein/lipid interactions. García-Sáez AJ, Schwille P. Methods. 2008 Oct;46(2):116-22.
  • Equinatoxin II permeabilizing activity depends on the presence of sphingomyelin and lipid phase coexistence. Schön P, García-Sáez AJ, Malovrh P, Bacia K, Anderluh G, Schwille P. Biophys J. 2008 Jul;95(2):691-8.

2007

  • Effect of line tension on the lateral organization of lipid membranes. García-Sáez AJ, Chiantia S, Schwille P. J Biol Chem. 2007 Nov 16;282(46):33537-44.
  • Single molecule techniques for the study of membrane proteins. García-Sáez AJ, Schwille P. Appl Microbiol Biotechnol. 2007 Aug;76(2):257-66. E
  • Pore formation by a Bax-derived peptide: effect on the line tension of the membrane probed by AFM. García-Sáez AJ, Chiantia S, Salgado J, Schwille P. Biophys J. 2007 Jul 1;93(1):103-12.

2006

  • Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores. García-Sáez AJ, Coraiola M, Serra MD, Mingarro I, Müller P, Salgado J. FEBS J. 2006 Mar;273(5):971-81.

2005

  • Peptides derived from apoptotic Bax and Bid reproduce the poration activity of the parent full-length proteins. García-Sáez AJ, Coraiola M, Dalla Serra M, Mingarro I, Menestrina G, Salgado J. Biophys J. 2005 Jun;88(6):3976-90.

2004

  • Membrane-insertion fragments of Bcl-xL, Bax, and Bid. García-Sáez AJ, Mingarro I, Pérez-Payá E, Salgado J. Biochemistry. 2004 Aug 31;43(34):10930-43.

2002

  • Peptides in apoptosis research. Salgado J, García-Sáez AJ, Malet G, Mingarro I, Pérez-Payá E. J Pept Sci. 2002 Oct;8(10):543-60. Review.
last update: February 23,2021